Introduction: COVID-19 continues to be a global health problem, as it is a virus that continues to evolve and vaccination continues to present obstacles. It is necessary to evaluate the knowledge about COVID-19 of the working population, since these allow supporting decision-making in favor of prevention. Objective:Validate a scale that measures knowledge about COVID-19 that the working population of Peru has. Material and Methods: A scale of 10 questions was made, submitted to expert judgment, evaluated in a pilot, and a survey of 663 workers was carried out. Results: Aiken’s V was greater than 0.7 in all items. A total of 9 items were obtained. Item 3 was found to be the most difficult (ID = 63%). Cronbach’s alpha was 0.701 (0.65-0.74). Conclusions: An instrument was validated, which can effectively measure workers’ knowledge about COVID-19. © 2022 Elsevier B.V., All rights reserved.